S. cerevisiae MAK-1 has also been previously used in simul-taneous remediation-detoxification and bioethanol productionprocess; this strain was cultured in shake-flask experiments onsugar-enriched pasteurized grape must in which the fungicidequinoxyfen had been added in various concentrations. Non-negligible amounts of microbial mass had been synthesized(∼10.0 g L−1) regardless the addition of fungicide into the medium.Ethanol was accumulated into the culture medium in very high con-centrations (∼106–119 g L−1) whereas the fungicide was efficientlyremoved from the medium to a rate of ∼79–82% (w/w) (Sarriset al., 2009). Significantly higher initial carbohydrates amountswere employed (initial sugars quantities at ∼260 g L−1) as com-pared with the present study. The medium that was used (grapemust enriched with glucose and fructose) was considered as idealfor growth and bioethanol production of S. cerevisiae MAK-1. Thus,it appears that not high initial total sugars concentration but poten-tially high melanoidin (and, in essence, high phenolic compounds)content negatively affected the strain’s metabolism in the presentstudy. Finally, comparing non-aseptic bioreactor with shake-flaskfermentations, biomass production was reduced in reactor culturesby means of both Xmaxand YX/TSvalues.