Subsequently, the effect of temperature was investigated in the
continuous ethanol fermentation of thick juice. The temperature of
R3 was controlled at 30 C, while the temperatures of R1 and R2
were increased from 30 C to 35 C. As shown in Table 2, the difference
of ethanol concentration in R1 was not significant when the
temperature increased from 30 C to 35 C (P ¼ 0.057). The ethanol
concentration in R2 at 33 C was not significantly different from
that at 30 C (P ¼ 0.414), while it significantly decreased to
75.2 g L1 at 35 C (P ¼ 0.016). Thus, the temperature in R2 should
be controlled at 33 C