Samples from activated sludge collected from the aerated basins
of a modified Ludzack-Ettinger (MLE) process that receivespretreated wastewater from an oil refinery industry located in La
Plata city, Buenos Aires, Argentina (345501700S, 575701600W), were
screened for bacteria that degrade oil derivatives. Ten ml of activated
sludge aliquots were inoculated in 90 ml E2 minimal medium
(Lageveen et al., 1988) supplemented with 20% kerosene as sole
carbon source and incubated at 300 rpm and 32 C for 7 days. After
incubation, culture aliquots were plated on LB agar. Bacteria with
different colony morphology were selected and picked in E2 agar
plates with diesel or kerosene as sole carbon source to verify
hydrocarbon utilization. To test for PHA accumulation isolates were
plated in E2 supplemented with 0.25% sodium octanoate and 0.2%
citric acid.
Samples from activated sludge collected from the aerated basinsof a modified Ludzack-Ettinger (MLE) process that receivespretreated wastewater from an oil refinery industry located in LaPlata city, Buenos Aires, Argentina (345501700S, 575701600W), werescreened for bacteria that degrade oil derivatives. Ten ml of activatedsludge aliquots were inoculated in 90 ml E2 minimal medium(Lageveen et al., 1988) supplemented with 20% kerosene as solecarbon source and incubated at 300 rpm and 32 C for 7 days. Afterincubation, culture aliquots were plated on LB agar. Bacteria withdifferent colony morphology were selected and picked in E2 agarplates with diesel or kerosene as sole carbon source to verifyhydrocarbon utilization. To test for PHA accumulation isolates wereplated in E2 supplemented with 0.25% sodium octanoate and 0.2%citric acid.
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