Negative results were reported by Toyosaki
et al. [18] and Navarro and Komagata [26] for some strains of
Gluconacetobacter xylinus, but Son et al. [15] and Kojima et
al. [8] reported positive reactions for certain isolates and
reference cultures. Isolated strains were all able to grow on
mannitol agar, whereas only AS14 showed scant growth on
glutamate agar. Even though the reference strains DSM
46604 and DSM 5602 were not able to grow on glutamate
agar, Navarro and Komagata [26] had previously reported
certain strains of Gluconacetobacter xylinus, which could
exhibit positive growth on glutamate. The isolated strains
could produce acids from glucose, sucrose, mannose,
galactose and xylose. The strain AS7 was distinguished from
others by acid production from fructose.