Samples of 5 mL were taken at 12, 24 and 60 h, which corresponds to the logarithmic, stationary, and late stationary phases of growth of each strain, and were centrifuged at 8000 rpm.The supernatants (extracellular fraction) were sterilized in an autoclave.Then 200 ll was added to small bottomless
tubes placed on the surface overlaid with S. reilianum, and inoculated
in YEPD plates as described above. The plates were incubated
at 28 C for 120 h and observed every 12 h. The filtrates with antifungal
activity were those that inhibited the development of the
fungus. In this case, inhibition halos were observed, which were
measured in mm. For the control, 200 ll of PDB medium ware
added.