1. In order to investigate the separation applicability of the prepared
materials (AgFeO2, and AgFe2O@CTS), different thiols (dithiothreitol, glutathione, thiabendazole, and sulfamethizole) were tested.
2. Typically,10 μL of the prepared materials (AgFeO2 and AgFeO@CTS) were
added to 1 mL of each thiol solution separately (1 × 10-3 M)
2.1 After 10 min, the magnetic nanoparticles were separated using external magnets and washed two times to removed unattached molecules.
3. The separated materials were diluted with 10 μL deionized water.
4. Then it was divided to three portions; first one (2 μL) was spotted
directly to MALDI plate.
5. Second and third portions (2 μL) were mixed
with DHB and MA (2 μL, 50 mM), separately and then spotted in a
MALDI plate.
6. The spots were dried before the measurements.
7.The overall procedures were represented in Fig. 1B.
all procedures were represented in Fig. 1B.
1. In order to investigate the separation applicability of the preparedmaterials (AgFeO2, and AgFe2O@CTS), different thiols (dithiothreitol, glutathione, thiabendazole, and sulfamethizole) were tested. 2. Typically,10 μL of the prepared materials (AgFeO2 and AgFeO@CTS) wereadded to 1 mL of each thiol solution separately (1 × 10-3 M)2.1 After 10 min, the magnetic nanoparticles were separated using external magnets and washed two times to removed unattached molecules.3. The separated materials were diluted with 10 μL deionized water.4. Then it was divided to three portions; first one (2 μL) was spotteddirectly to MALDI plate. 5. Second and third portions (2 μL) were mixedwith DHB and MA (2 μL, 50 mM), separately and then spotted in aMALDI plate. 6. The spots were dried before the measurements. 7.The overall procedures were represented in Fig. 1B.all procedures were represented in Fig. 1B.
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