2.5. Antibacterial assessment
Antibacterial activity of EHCs against E. coli was evaluated
by using optical density method described as follows:
A representative bacteria colony was picked off, placed in a
nutrient broth (peptone 10 g, beef extract 3 g, NaCl 5 g in
distilled water 1000 ml; pH 7.0–7.2) and incubated at
37 C for 24 h. Then the obtained fresh culture where