The Agilent 1200 analysis HPLC system (Agilent Technologies, Santa Clara, CA, USA) was fitted with an additional pump to supply the ABTS radical solution.
The ABTS solution was prepared with the same method as that used for off-line. An aliquot of 10 ll black bamboo extract methanol solution (10 mg/mL) (40 C and 200 C
with PLE) was injected into the on-line HPLC-ABTS+ system.