26.9mmol/g respectively, while the overloaded solanesol concen-tration was 15mM corresponding to 20mM sample concentration, indicating good specificity of MIPs to solanesol. Compared with the reported solanesol MIPs [28,29] in irregular shape and mem-brane with adsorption capacity as 58.12mg/g, the spherical MIPs prepared in this study were superior in adsorption performance and morphology as chromatographic stationary phase. In order to obtain pure solanesol, the pretreated crude methanol extract of dry tobacco leaves (20mM, 40ml) was loaded, leached and eluted under optimized preparation chromatographic system con-ditions. The chromatogram and its corresponding HPLC analysis were shown in Fig. 4