16S-23S IGS region ampli fication of Vibrio sp: Al l
oligonucleotide primers used in this study were synthesized by
Bionics, Korea. The PCR primers used for amplification of DNA
fragments spanning the 16S-23S IGS regions were targeted at highly
conserved regions of the 3' and 5' ends of the 16S and 23S rDNAs
respectively. 16S-23S IGS regions was amplified and analyzed
according to Lee et al.(2002). Species-specific primers for detection
of the 5 Vibrio sp are given in Table 1.