The highest unsaturated fatty acid in Artemia sp.
after feeding with Chaetoceros calcitrans was oleat
(25.09%). Oleate is the substrate that forms the long
PUFA fatty acid chain. Pratiwi et al. (2009) stated that
oleate is the substrate in the process of denaturation
and catalyst elongation. PUFA biosynthesis starts
from oleate, and then into linoleum as a substrate for
the formation of the omega 6 long chains, and finally
into linolenum that functions as the substrate for the
formation of the omega 3 long chains. The process
of PUFA formation involves a series of denaturation
processes and elongation of catalysts with the help
of desaturation and elongation enzymes (Fabergas et
al. 2009).