2.4. SDS–PAGE (Polyacrylamide gel electrophoresis)
The protein analysis of the isolates that showed 100% mortality at day 5, was achieved through protein electro-phoresis in polyacrylamide gels (SDS–PAGE 10%). The proteins were extracted from the culture according toLeca-det et al., 1991. The gel received 15ll of each sample. Afterwards, the gel was stained and fixed in 40% methanol; 10% acetic acid and Coomassie blue (0.1%) for about 16 h, under slight shaking and it was destained in 40% methanol and 10% acetic acid for 2 h, with agitation