2.7 Antimicrobial activity of LDPE-EVA films
The different types of the films as well as the film without EO (control film) were tested for their antibacterial ratio the selected microorganism E. coli (Gram-negative bacteria) and S.aureus (Gram-positive bacteria) by dynamic shake flask test method (ASTM E2149-10). Four grams of film samples were immersed in 50 ml of nutrient broth and subjected to sterilization by autoclave after that inoculated by an initial concentration of bacteria of 108 CFU/ml. The samples were incubated at 37oC for 24 h. The final concentration of CFU was determined by bacteria plate counting on plate count agar medium from 10-fold serial dilutions of the bacteria suspension. Finally evaluate bacterial survival and antibacterial ratio from equation 1 and 2 respectively.