Plant materials: The fresh flower species were collected
and authenticated from department of Botany, YC College
of Science and Engineering, Satara. Collected leaves of
Acalypha wilkesiana cleaned with water and cut into very
small pieces by chopping blender. The juice is stained off
from the resulting mush also extracted with water to yield
more pigment. Finally solution is filtered to remove
remaining plant matter and used as natural indicator.