Referring to the difficulty in
obtaining an aseptic in vitro culture starting from a woody species, the average number of
healthy explants obtained after the disinfection of foliar explants of Annona sp. using the antimicrobial
substances tested was significant. Further studies to evaluate the effects of the concentration
of chemicals on in vitro plant regeneration for Annona species are needed to clarify
the relationship between the concentration and phytotoxic effect of chemicals.