Rest of the 90 μl of amplified reaction mixture was submitted to M/S Xcelris
Labs Limited, Ahmedabad for custom sequencing (both strands) of amplified DNA
for both strands using either of the two 16s rRNA forward or reverse primer in a
single sequencing. The final sequence of all the amplified 16S rRNA genes were
edited using DNA software Chromaslite 201, and CLC Sequence Viewer 6.5.4 (CLC
bio A/S)