suggested the rat CMAP assay as a method for quantifying
BoNT.The results included linearity within the range of
0.01–30 U/head on day 1 (R2 ¼ 0.979) and 0.01–10 U on
subsequent days (R2 ¼ 0.971 on day 2, R2 ¼ 0.966 on day 4,
R2 ¼ 0.954 on day 7, R2 ¼ 0.959 on day 14, respectively), and
reproducibility between the two measurers (Sakamoto
et al., 2009).