However, at the same extraction conditions, the α-chains and cross-linked components of gelatin from seabass skin were more tolerant to thermal hydrolysis than those of brownbanded bamboo shark and blacktip shark. Gelatins from splendid squid skin with higher extraction temperatures contained a lower band intensity of the α-chains than those obtained with lower extraction temperature (Nagarajan et al., 2012). Generally, gelatins with a higher content of α-chains showed better functional properties including gelling, emulsifying and foaming properties geometry used was a 3.5 cm parallel plate and the gap was set at 1.0 mm. The measurement was performed at a scan rate of 0.5 ﹾC/min, frequency of 1 Hz, oscillating applied stress of 3 Pa during cooling from 35 to 5 ﹾC and heating from 5 to 35 ﹾC. The gelling and melting temperatures were calculated, where tan δ became 1 or δ was 45ﹾ.