During exsanguination, blood samples were collected directly into
sterile 10 ml Vacutainer tubes and placed on ice. Two samples were
taken fromeach of 160 animals (80 control and 80AMtreated animals).
Vacutainer tubes containing EDTA as an anticoagulant at a proportion of
2 mg/ml of blood were used for the analyses of blood cellular components
(hematocrit value, hemoglobin content, erythrocyte and leukocyte
counts), while Vacutainer tubes without anticoagulant were used
for the analyses of blood biochemical components (glucose, lactate
and cortisol). The blood for the analyses of the biochemical components
was centrifuged at 2500 rpm for 10 min (room temperature) and
the serum was separated into 5 ml sterile vials and stored at 0 °C until
analysis. The samples used for the analyses of cellular components
were stored at 4 °C until required. All samples were processed at the
Laboratory of Clinical Pathology, Faculty of Veterinary Medicine of the
National Autonomous University of Mexico.
During exsanguination, blood samples were collected directly intosterile 10 ml Vacutainer tubes and placed on ice. Two samples weretaken fromeach of 160 animals (80 control and 80AMtreated animals).Vacutainer tubes containing EDTA as an anticoagulant at a proportion of2 mg/ml of blood were used for the analyses of blood cellular components(hematocrit value, hemoglobin content, erythrocyte and leukocytecounts), while Vacutainer tubes without anticoagulant were usedfor the analyses of blood biochemical components (glucose, lactateand cortisol). The blood for the analyses of the biochemical componentswas centrifuged at 2500 rpm for 10 min (room temperature) andthe serum was separated into 5 ml sterile vials and stored at 0 °C untilanalysis. The samples used for the analyses of cellular componentswere stored at 4 °C until required. All samples were processed at theLaboratory of Clinical Pathology, Faculty of Veterinary Medicine of theNational Autonomous University of Mexico.
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