The experiment was conducted following the
guidelines for animal care existing at the Tokyo
ARTICLE IN PRESS
A. Panigrahi et al. / Developmental and Comparative Immunology 31 (2007) 372–382 373
University of Marine Science and Technology. The
fish were reared in 60 L tanks arranged in a flowthrough
system; every treatment was done in
triplicate. Each tank was stocked with 15 rainbow
trout (av. weight 94 g), which had been grown
previously on commercial feed. The fish were
offered the basal control diet for a 2-week conditioning
period. After this they were fed the
experimental diets, two times daily to satiation, for
a period of 45 days. The water temperature
averaged 1271 1C throughout the experiment.