Standard curves of the optimized assays using SYBR Green I were
created. No primer dimers with lower T
values were observed. When
data were compared to HS groups, a significantly greater change in
intestinal bacterial composition was observed in the IBD group (Fig. 1,
Table 3). A statistically significant decrease in the numbers of
Bacteroides–Porphyromonas–Prevotella was observed in both the A-
m UC (7.03) and A-CD groups (5.52) (P = 0.014 and P = 0.003,
respectively). These values, however, showed no significant difference
between the HS (8.49) group and the R-UC group (8.33)
(Table 3).