Analysis of extracted samples by thin layer chromatography showed the presence of lycopene as well as α and β-carotenoids. HPLC was done to determine the concentration and purity of extracted lycopene. The optimal mobile phase for HPLC analysis was found to be 3:1 (v/v) acetonitrile: dichloromethane. It gave a good chromatographic response and efficient separation of lycopene within a short retention time.