This research aims to study the anti-pathogenic bacteria and fungi in the skin of the volatile oil from lavender and Lemongrass House. By extracting the oils by refining methods with water, then bring it back for the chemical composition of the essential oil with the gas way chromatography-mass spectrometry (GC-MS) test, anti free radicals with thiobarbituric acid reactive substances method (TBARs), and test the minimum concentration can inhibit infections.Bacteria and fungi (MIC) broth dilution method with method of concentration as low as it can kill the bacteria (MBC) and the lowest concentration that can kill fungi (MFC) from the results of this research have found that lemon grass, citronella and House the output percentage of the essential oils, equivalent to 0.35 and 0.21 respectively. Analysis of the chemical composition of the essential oil with the GC-MS method, found that citronella essential oils find all chemical elements 11 elements found Geraniol (30.23 percent). Essential oils from lemon grass House. Takeda chemical elements found all the elements discovered by Citral (52.42 percent). Test results for anti-free radicals with TBARs method found that essential oils from lemon grass, Lemongrass and home at concentrations of 10 mg/ml. Can lip Super rapid oxidation reaction equal to ± 0.005 and 0.001 61.778 ± 64.833, respectively, but both types of essential oils is to inhibit the rapid oxidation reaction occurring lip less than Super BTH equals 84.854 ± 0.006 inhibition bacteria test results of citronella essential oils? It found that Staphylococcus aureus bacteria can inhibit Staphylococcus epidermidis and is equal to the MIC and 1.953 per microliter 0.976 ml, respectively, and the Candida albicans fungus microbes and Trichophyton mentagrophytes 0.488 MIC and charge per microliter 0.061 ml. The section from lemon grass essential oil can inhibit S. aureus bacteria and S. epidermidis MIC charge equal to 62.5 per microliter ml and can inhibit the fungus C. albicans and T. Mentagrophytes MIC charge equal to 0.061 microliter per ml, and test results for the influence to kill the bacteria (MBC), and fungi (MFC)Essential nganamman from citronella Found that S. aureus, and the bacteria can kill the S. epidermidis MBC charge equal to 125 and microliter per ml, respectively and 15.625 can kill fungi, C. albicans, and T. Mentagrophytes by has a value the same MFC is 7.812 per ml, respectively, and 0.122 microliter. The section from the Lemongrass essential oil can kill the bacteria S. aureus and S. epidermidis MBC charge equal to 62.5 and 31.25 per microliter ml, respectively, and can kill fungi, C. albicans, and T. Mentagrophytes by has a value the same MFC is 7.812 per microliter 1.953 and ml.