2. Materials and methods
2.1. Raw material (substrate)
The method of juice extraction, its treatment
and chemical composition were described by
Nigam (1999).
2.2. Carrageenan
Carrageenan used for the cell immobilization
was obtained from the (Kobenkavns Rektinfabrik,
Denmark). It was a Genugel type X-0828
especially developed for immobilization
procedures.
2.3. Microorganism and culture conditions
The maintenance and culture conditions of
Saccharomyces cere6isiae ATCC 24553, were described
by Nigam (1999). The cells were harvested
in the late exponential growth phase and
concentrated.