Last Chance for Animals seeks to eliminate animal exploitation through direct action, education, investigations, legislation, and media attention. LCA opposes the use of animals in food and clothing production, scientific experimentation, and entertainment. LCA also promotes a cruelty-free lifestyle and the ascription of rights to non-human beings. They support veganism and oppose animal testing.
As their budget and staff increased from 2000 and on, LCA has expanded its focus beyond vivisection. LCA also now focuses on pet theft, the fur trade, and circuses, and it has also focused on animal use in other forms of entertainment, as well as on large-scale "factory" farming issues. The organization also provides money and support for the animal rescue mission occurring in areas heavily affected by the 2011 tsunami in Japan.