In this study, the ability of nine different protease preparations to deproteinize shrimp waste for chitin extraction was investigated. B. mojavensis A21 and B. capriscus proteases, which were able to eliminate up to 77 ± 3% of the shell proteins using with an E/S ratio of 5, were selected for chitin extraction. Chitin samples were then characterized and compared to sample prepared through chemical deproteinization.
The biological activities of chitosan-M and chitosan-C obtained by enzymatic and alkaline treatment, respectively, were studied. Chitosan-C with lower MW (5.820 g/mol) exhibited higher antioxidant activity. Further, chitosan-C exhibited higher antitumor activity on bladder carcinoma cells (RT112) and showed slightly higher antimicrobial activity than chitosan-M with higher MW (19,780 g/mol). The significance of this effect must be investigated further across a wide variety of chitosan molecular weights. Nevertheless, these important properties make chitosan an exciting and promising excipient for agriculture, packaging and pharmaceutical industry.
In this study, the ability of nine different protease preparations to deproteinize shrimp waste for chitin extraction was investigated. B. mojavensis A21 and B. capriscus proteases, which were able to eliminate up to 77 ± 3% of the shell proteins using with an E/S ratio of 5, were selected for chitin extraction. Chitin samples were then characterized and compared to sample prepared through chemical deproteinization.The biological activities of chitosan-M and chitosan-C obtained by enzymatic and alkaline treatment, respectively, were studied. Chitosan-C with lower MW (5.820 g/mol) exhibited higher antioxidant activity. Further, chitosan-C exhibited higher antitumor activity on bladder carcinoma cells (RT112) and showed slightly higher antimicrobial activity than chitosan-M with higher MW (19,780 g/mol). The significance of this effect must be investigated further across a wide variety of chitosan molecular weights. Nevertheless, these important properties make chitosan an exciting and promising excipient for agriculture, packaging and pharmaceutical industry.
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