Microscopy
M. truncatularoots growing on agar were covered
with bioFolie 25 (Sartorius AG, Vivascience Support
Center, Go¨ttingen, Germany) and observed with a
LEICATCS 4D confocal microscope (Leica, Germany)
using a 63water-immersion objective. Four-day-old
A. thalianaseedlings were mounted in liquid half MS
medium containing 1% sucrose using a spacer of one
layer of parafilm between slide and coverslip, and
observed by confocal microscopy. Plants were adapted
to liquid medium overnight to allow application of
drugs.
For the observation of transgenicA. thalianaexpressing Ara6-GFP (Ueda et al., 2001), serial images were
obtained every 1mm using a fluorescence microscope
(Olympus, Japan) equipped with 40oil-immersion
objectives and a confocal unit, CSU10 (Yokogawa
Electric Corporation, Japan). Images and movies were
digitally processed with Image-Pro Plus 4.1 (Media
Cybernetics, L.P.), Adobe Photoshop 4.0 (Adobe Corp.,
Mountain View, CA) and VideoMach 2.7.2.