V. Appendix B: Composition and Preparation of Common
Buffers and Solutions
A. Preparation of Bicarbonate-Carbonate Buffer (pH 9.2–10.8)
To create 100ml of a 0.1M bicarbonate buffer solution, mix
sodium bicarbonate and sodium carbonate, decahydrate,
as given below.
Solution A: 0.1M sodium bicarbonate (NaHCO3 MW = 84.0)
(MW = molecular weight)
Solution B: 0.1M sodium carbonate, decahydrate
(Na2CO3•10H2O FW = 286.2) (FW = formula weight)