Following thermal sealing with pierceable foils in a plate sealer
PXI (Bio-Rad, foil Cat. No. 181-4040), the following temperature
profile was applied as standard for PCR cycling: 600 s at 95 C, 45
cycles of 15 s at 95 C, and 60 s at 60 C. Following PCR, the sealed
plates were processed in the droplet reader of the QX100 system
(Bio-Rad), and the droplets analyzed according to manufacturer's
recommendations (Droplet Reader Oil Cat. No. 186-3004)