Direct immunofluorescent testing of ulcer material using an H ducreyi-specific monoclonal antibody appears to be useful (8,9). Hansen et al (10) developed an antigen detection assay to detect H ducreyilipooligosaccharide (LOS) using an LOS-specific monoclonal antibody and an adaptation of the limulus amoebocyte assay. The sensitivity and specificity of these antigen detection methods are 89% to 100%, and 63% to 81%, respectively (8). However, the reagents for these two antigen detection methods are not commercially available. Ulcer specimens for DFA may be collected, air dried and fixed until a reference laboratory can be identified to perform DFA.