The animals were then returned to their cages. After
24 hours, the bandage and the test materials were
removed and 1 hour later the sites were examined for
skin irritation. Observation of the sites was done at 24
hours after application, and repeated at 48 and 72 hours
thereafter. The reactions, defined as erythema and
edema, were evaluated according to the scoring system
for skin reactions (Table 2).