Place vibratome sections mounted on slides into 4% glutaraldehyde
in 0.1 M cacodylate buffer with sucrose for 10 min (tissue
previously in phosphate buffer must be rinsed first to avoid precipitate).
For 1 liter of buffer: 21.4 g of sodium cacodylate are mixed
and brought to 1000 ml distilled water. Adjust pH to 7.4 using 1 N
HCl. Osmolarity is 350 mOsm and solution is filtered and kept in
refrigerator. Add 4% glutaraldehyde by dilution of 25 to 50%
ampules into buffer on day of use for appropriate volume.