Enzymatic hydrolysis was performed using commercial solution
of Trichoderma reesei cellulase (ACCELLERASE® 1500) of activity
97.4 FPU/mL. The enzyme concentration used was 40 FPU/(g d-b).
The hydrolysis tests were done at 55 C for 72 h using 1% dry matter
in 200 mL Erlenmeyer flasks. The preparation of the 1% dry matter
suspension was done by measuring moisture content of the pretreated
residues. Then, the wet basis equivalent of 1 g dry matter
was weighed and the enzyme added. The mixture was then
completed to 200mL with 50mMcitrate buffer (pH 4.5) containing
1% sodium azide (2%) [30]. In parallel, three types of controls were
set: the first contained 1 g of non-pretreated matter with citrate
buffer and the enzyme. The second and third respectively contained
1 g of pretreated matter and 1 g of non-pretreated matter in
citrate buffer without enzymes. The experiments were done in
triplicate.