Commercial Second-generation sequence (SGS) emerged in 2005 in response to the low throughput and high cost of first-generation methods. To address this problem, SGS tools achieve much higher throughout by sequencing a large number of DNA molecules in parallel. Several reviews of what was first called “next-generation” or, more precisely, SGS technologies have appeared and this technique was classified as a combination of a synchronized reagent wash of nucleoside triphosphate (NTPs) with a synchronized optical detection method.