ABSTRACT The choice of K2- or K3-ED

ABSTRACT The choice of K2- or K3-EDTA as the preferred anticoagulant for blood count remains controversial. We compared the effect of different concentrations of both anticoagulants on normal blood. In optimal conditions (appropriate anticoagulant concentration and measurements done between 1 and 4 h after phlebotomy), no marked differences are seen between either EDTA salt. Important discrepancies appear, however, in less optimal conditions, as often happens in day to day practice. The packed cell volume, when measured on centrifuged blood, decreases with increasing anticoagulant concentrations and this is most pronounced with the K3 salt. This phenomenon has been reported by different authors and is ascribed to shrinking of erythrocytes in an hypertonic medium. Automated instruments react in a different way, their MCV is not influenced by K3-EDTA concentrations up to ten times normal, while K2-EDTA, at high concentrations, results in a slight increase in MCV, as measured with three of the instruments. With most instruments, the accuracy of the white cell count is not markedly influenced. However, when measured with the Unipath CD 3000 all tested blood samples, taken in high K3 (but not in K2) concentrations, showed an appreciable decrease in the leucocyte count (to less than 50% of the original value, at a concentration of 15 g/l, 24 h after blood collection). Measurement of RDW and automated differentials is also influenced by the choice of anticoagulant when determinations are done in less than optimal conditions. We conclude that the choice of anticoagulant, its use at an appropriate concentration and the age of the blood sample are important matters and should be given due consideration.(ABSTRACT TRUNCATED AT 250 WORDS)

ผลลัพธ์ (คลิงออน) 1:

k2-qoj k3-edta wIv ABSTRACT Hoch maS anticoagulant 'Iw 'ech ratlh controversial. 'angbogh Da pIm concentrations cha' tlhIH anticoagulants motlh 'Iw compared maH. qaStaHvIS optimal je (appropriate anticoagulant concentration 'ej measurements Qu' DuqIppu'chugh 1 4 h qaSpu'DI' phlebotomy), legh pagh ghItlh differences SabtaHbogh either edta salt. nargh potlh discrepancies, 'ach pa' qup optimal je, Hoch pIj qaS qaStaHvIS jaj jaj qeq. ghaH anticoagulant concentrations ghur nup pack cell muq, HeghDI' 'aD centrifuge 'Iw 'ej 'ach pronounced ghu'vam je k3 salt. ja' pong pIm authors 'ej ascribed shrinking hypertonic medium erythrocytes wanI'. qaStaHvIS mIw pIm SoQHommey automate jan, wej moH mcv pong k3-edta concentrations Da'elDI' wa'maH poH motlh, k2-edta, poStaHvIS DeSDu' jen concentrations, ghot'e' neH loQ ghur pa' mcv, Hoch 'aD wej jan. ghaH HochHom jan, wej markedly moH chIS cell 'ech accuracy. 'ach HeghDI' 'aD unipath cd 3000 Hoch waH 'Iw samples, tlhap neH jen k3 ('ach wej je k2) concentrations, appreciable nup neH leucocyte 'ech (qup HoS 50 vatlhvI' original lo'laHghach, DeSDu' concentration 15 g ghap l, 24 h qaSpu'DI' 'Iw vuDmey tlhopDaq) 'ang. je moH measurement rdw automate differentials 'ej pong anticoagulant wIv HeghDI' Qu' determinations qaStaHvIS qup HoS optimal je. vaj qay' potlh 'ej vaj due anticoagulant wIv legh appropriate concentration 'Iw sample qantaHvIS je lo', conclude maH tlhoQ. (ABSTRACT TRUNCATED DESDU' 250 MU')