An electromagnetic lithotripter (MODULITH SLK device Storz
Medical AG, Switzerland) was used throughout the present study.
· 106
cells were cultured in 50 mm Petri dishes in 2 ml medium and
reated with ESW directly focusing the centre of the plate under eco-
raphic control. In order to measure NO production, 1.5 · 105
cells
were plated on glass coverslips one day before ESW treatment. After
ESW treatment, cells were maintained in humidified atmosphere of
5% air plus 5% CO2 at 37 C for the time indicated in each experi-
ment.