In this paper, we studied the interaction between baicalin/baicalein and the s-BLM on Pt electrode by cyclic voltammetry with Fe(CN)63 − as probe ions. The electrochemical results show that baicalein could induce much more defects in the s-BLM. Further UV–Vis spectroscopy studies indicate that the baicalein molecule can insert in the core of lipid bilayer membrane. The better transmembrane ability of baicalein could make it easier to develop bioactivity of Radix Scutellariae than baicalin. The methods developed in this paper can be used as preliminary in vitro models to study and screen bioactivity of drugs on their abilities of passive diffusion through biomembranes.
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