Results and Discussion
To account for changes in mass created by chemical
The obiectives of the research renorted here are twofold: The first is to develop a computer algorithm
to search a protein database by using uninterpreted
tandem mass spectra from a single peptide. No additional
information about the peptide beyond the mass
of the peptide is used. In this manner unrelated peptides
generated with proteases of unknown specificity
can be analyzed, and if the amino acid sequence is in
the protein database, an interpretation of the tandem
mass spectrum can be performed. The second objective
is to test the method on representative peptides of
known and unknown sequence (unknown to the researcher)
to determine the effectiveness of tandem
mass spectrometry data to identify the origin of the
sequence.