were determined by dynamic light scattering (DLS) (Zetasizer, Malvern
Instruments, UK). Each sample was diluted with deionized water,
adjusted to a suitable light scattering intensity (300 Hz) and measured
at 25 C in triplicate. Following the particle size analysis of
the cubosomes, the mode was switched from ‘‘Size’’ to ‘‘Zeta,’’
and three measurements of zeta potential were recorded.