Our previous studies have revealed that the Tricholoma lobayense polysaccharide TLH-3, which has an IC50 value for scavenging superoxide radicals comparable to that of Vitamin C (VC), could be prepared by hot water extraction and column chromatography. However, this method is tedious and in efficient. In this study, high-pressure homogenization (HPH) was used to extract polysaccharides from T. lobayense
Heim. The yield of 17.3% using HPH was higher than the 12.3% yielded by traditional extraction. More-over, the operation combined quaternary ammonium salt precipitation with ultrafiltration (QASP-UF) to purify
the crude polysaccharides successfully, which provided a higher TLH-3 yield, a larger
separation volume, and a shorter purification time than that of column chromatography. The whole process for TLH-3 preparation presented here exhibited significant advantages
of quicker processing, low consumption,high efficiency, and flexible compatibility with follow-up studies. Based on a characterization of molec-ular weight, Fourier Transform Infrared Spectroscopy (FT-IR) analysis, and monosaccharide composition
analysis, it can be concluded that TLH-3 and TLH-3 are the same polysaccharide. Antioxidant activity was tested to prove that the TLH-3 prepared in this study could scavenge DPPH and superoxide radicals, with IC50 values of 111.7 g/mL and 160.0 g/mL, respectively. Its outstanding antioxidant ability was comparable to that of ascorbic acid.