Differentiation of 3T3-L1 preadipocyte in the presence of
a-tomatine
3T3-L1 preadipocyte cells were differentiated into adipocyte
cells as already reported (Student, Hsu, & Lane, 1980). The induction
medium containing a-tomatine or the extract of immature
green tomatoes was added to the confluent cells and was incubated
for 6 days. The total volume of medium was 500 lL in a
12-well plate, and the volume of a-tomatine or the extract of
immature tomatoes was from 5 to 20 lL.