At each time interval, the elastomeric modules (Ormco, Orange, Calif) were carefully removed to disengage the archwires by the same operator. Plaque specimens were collected from the labial surfaces immediately surrounding the orthodontic brackets of the maxillary lateral incisors with a sterilized scaler using a 4-pass technique as suggested by Pellegrini et al.20 Four passes, each along the tooth at the bracket interface at the gingival, mesial, distal, and occlusal aspects, were used to prevent overloading the instrument tip.
At each time interval, the elastomeric modules (Ormco, Orange, Calif) were carefully removed to disengage the archwires by the same operator. Plaque specimens were collected from the labial surfaces immediately surrounding the orthodontic brackets of the maxillary lateral incisors with a sterilized scaler using a 4-pass technique as suggested by Pellegrini et al.20 Four passes, each along the tooth at the bracket interface at the gingival, mesial, distal, and occlusal aspects, were used to prevent overloading the instrument tip.
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