The filling procedure was executed with four
replicates per injection which resulted in 24 different injection
times of the enzyme on one microplate. The ascorbic
acid concentrations for this experiment were 215mg L−1 for
the oxidase-method, 550mg L−1 for the OPDA-method, and
17 mg L−1 for the PMS-method in order to obtain comparable
absorbance readings in the spectrophotometer