ABSTRACTOur previous study demonstrated that the aqueous extract from freshwater macroalgaRhizoclonium hieroglyphicum (C.Agardh) K tzing (RW) contained mostly polysaccharides and aminoacids which might play an important role as a skin moisturizer. Therefore, its moisturizingeffect in pig skin model and human skin was compared with some well-known commercialmoisturizers such as glycerin (G), propylene glycol (PG) and hyaluronic acid (HA). The RWcream was then formulated and investigated its physical properties, pH and viscosity as well asstability under various storage conditions. The skin irritation and clinical evaluation for skinmoisturizing effect in healthy volunteers were also evaluated. Skin patch test using Finn chamber®and skin moisturizing measurement using Corneometer® were conducted in 30 healthy volunteers.The skin hydration was measured at before and after applying the RW cream and controlcreams (cream base, G, PG and HA creams) for 15, 30 min, 1 hour for a short-term effectand 1 week (twice daily) for long-term usage. Satisfaction test using questionnaire was alsoperformed. The results revealed that the moisturizing effect on human skin of RW is verysimilar to hyaluronic acid and glycerin. From clinical evaluation, the skin hydration increasedafter using RW cream in both short-term and long-term usage and no skin irritation occurred.Interestingly, the moisturizing effect of RW cream was comparable to HA cream (p<0.05).More than 80% of the volunteers were satisfied with RW cream. In conclusion, the freshwatermacroalga extract might be a promising natural skin moisturizer for skin care cosmetics.
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