4. Conclusions
This study has clearly demonstrated that lipid in Chlorella sp.
can be effectively extracted by EA/E. Lipid extraction was significantly
enhanced by increasing solvent to biomass ratio, extraction
temperature, extraction time. The fatty acid profiles of lipid
illustrated that the most abundant components were palmitic,
linoleic and linolenic acids, suggesting that the microalgae cultivation
in outdoor raceway ponds combined lipid extraction by EA/E
was a feasible process for microalgae based biodiesel production.
However, the optimal extraction conditions is still required to be
verified and economic analysis of the extraction process should be conducted in further study.