Optimization of culture condition for PBS depolymerase production
Different cultures conditions for maximum production of PBS depolymerase enzymes were optimized and investigated at different carbon source and nitrogen source in basal medium. PBS on basal medium with 0.1% (w/v) final concentration in 100 mMTris-HCl buffer (pH 7.0) was used as substrate for enzyme activity determination. All the experiments were performed in a 250 ml flask that has 20 ml of basal medium The inoculated medium was incubated with rotary shaking at 150 rpm for 4 days. The culture medium was centrifuged at 12,000 rpm for 10 min to obtain the crude enzyme extract and used for analyzing enzyme activity. Effect of carbon sources, enzyme production was observed at various carbon sources of 0.1% (w/v) sucrose, glucose, fructose, PLA, PBS and PBSA. Effect of nitrogen sources, various nitrogen sources of 0.12% (w/v) such as yeast extract, ammonium sulphate, casein, gelatin and yeast extract with ammonium sulphate were examined