A recently established method for the germination of Echinochloa seeds recognised and accounted for variation
in responses to light and darkness. This method used parallel light and dark tests and was successful in
promoting germination in most seed lots. However, some samples exhibited deeper dormancy and were not
fully responsive to either test. In the present study, we employed warm pretreatments where seeds were exposed
to dilute aqueous ethanol solutions to attempt to break their dormancy. Based on tests of five Echinochloa
accessions, we propose a new, follow-up protocol that can be used on samples unresponsive to the established
method. The additional step involves a 3-day dark pretreatment at 35-37°C, imbibing the seeds in ~0.25M
ethanol (aq). After this pretreatment, the seeds should be germinated for 14 days at 20/30°C (16 hours/ 8 hours)
with half held in darkness and the other half exposed to an 8-hour light cycle
A recently established method for the germination of Echinochloa seeds recognised and accounted for variationin responses to light and darkness. This method used parallel light and dark tests and was successful inpromoting germination in most seed lots. However, some samples exhibited deeper dormancy and were notfully responsive to either test. In the present study, we employed warm pretreatments where seeds were exposedto dilute aqueous ethanol solutions to attempt to break their dormancy. Based on tests of five Echinochloaaccessions, we propose a new, follow-up protocol that can be used on samples unresponsive to the establishedmethod. The additional step involves a 3-day dark pretreatment at 35-37°C, imbibing the seeds in ~0.25Methanol (aq). After this pretreatment, the seeds should be germinated for 14 days at 20/30°C (16 hours/ 8 hours)with half held in darkness and the other half exposed to an 8-hour light cycle
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