fractions of corresponding allergens were
collected and freeze-dried in order to perform SDS–PAGE and immunoblotting tests. The best method
was identified the one with a shorter retention time, better resolution, and greater peak height as com-
pared with the other methods. In general, the peak heights were greater at 220 nm than at 280 nm. The
major disadvantage of the C12 column was the need for two sets of conditions to identify the allergens as
compared to the C18 column where all three allergens could be identified in one run