TX-114 extraction has been used most frequently for the isolation of proteins from animal tissues and to a lesser extend from plants. In only a few cases has it been applied to micro-organisms. The macromolecular components extracted and separated from bacterial species were quite varied, such as membrane-associated polypeptides
from Treponema pallidum, a lipopolysaccharide-like substance and proteins from Leprospira interrogans, pyruvate oxidase from Escherichia coli, a hydrogenase from Paracoccus denitrificans, a wall protein from Methanococcus voltae, and other antigens from Borrelia burgdorferi and Mycoplasma hyorhinis, proteins,lipoarabinomannan and phosphatidylinositols from Mycobacterium leprae and lipoteichoic acid from Streptococcus mutans.