2.3. Soluble protein content determination
Soluble protein content is the most important factor for physicochemical properties and correlates with functional properties such as emulsibility, foaming and gelation.
Hamid-Samimi and Swartzel (1985) suggested that 5% soluble protein loss was the maximum limit for producing a functionally acceptable product.
After PEF processing, the egg white solution was centrifuged at 10,000g at 4 C for 30 min.
Supernatant (5 mL) was collected and its protein content was determined by the Kjeldahl method, using a mixture of K2SO4, CuSO4 and Se (10:1:0.1) as catalyzer (AOAC, 2000).
The protein solubility of each sample was an average of triplicate analysis.